Interactive:
1. Used alone to tell somebody to stop doing what they are doing or not to do something. English = 'Don't!', 'Stop that!'
2. Used alone to mean that you think there is no possibility or likelihood of what is being discussed actually happening or being really true. English = 'No!', 'No, that's not right!', 'No way!', 'Absolutely not!'
As Modifier:
1. Used immediately next to a verb by some signers to negate the action of the verb. English = not, do not, does not, did not etc.